Detailed Abstract
[BP Poster Presentation 1]
[BP PP 1-4] Superior gallstone dissolubility and safety of tert-amyl ethyl ether over methyl-tertiary butyl ether
Dong Do YOU1, Tae Ho HONG2, Ho Joong CHOI2, Jung Hyun PARK5, Kee-Hwan KIM4, Jae Hyun HAN1, Kwang Yeol PAIK3, Say-June KIM*2
1Department of Surgery, St. Paul's Hospital, College of Medicine, the Catholic University of Korea, Korea
2Department of Surgery, Seoul St. Mary’s Hospital, College of Medicine, the Catholic University of Korea, Korea
3Department of Surgery, Yeouido ST. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Korea
4Department of Surgery, Uijeongbu St. Mary's Hospital, College of Medicine, the Catholic University of Korea, Korea
5Department of Surgery, Eunpeong St. Mary's Hospital, College of Medicine, the Catholic University of Korea, Korea
Introduction : The use of methyl-tertiary butyl ether (MTBE) to dissolve gallstones has been limited due to concerns over its toxicity. The adverse effects of MTBE are largely attributed to its low boiling point, resulting in a tendency to evaporate. Herein, we explored tert-amyl ethyl ether (TAEE), an MTBE analogue with a relatively higher boiling point (55 °C) and compared its efficacy and toxicity with those of MTBE.
Methods : The in vitro dissolubility was determined by measuring the dry weights of human gallstones after treating with each solvent. The in vivo dissolubility was determined by comparing the weights of solvent-treated gallstones and control (dimethyl sulfoxide)-treated gallstones, after the direct infusion of each solvent into the gallbladder of hamsters with gallstones.
Results : The in vitro results demonstrated a 24-h TAEE-dissolubility of 76.7%, 56.5 and 38.75% for cholesterol, mixed, and pigmented gallstones, respectively, which represented a 1.2-, 1.4-, and 1.3-fold increase in dissolubility compared to that of MTBE. In the in vivo experiment, the 24 h-dissolubility of TAEE was 71.7% and 63.0% for cholesterol and pigmented gallstones, respectively, which represented a 1.4- and 1.9-fold increase in dissolubility compared to that of MTBE. In addition, the cell viability assay and western blot analysis indicated that TAEE had a lower toxicity towards gallbladder epithelial cells than MTBE.
Conclusions : TAEE has higher gallstone dissolubility properties and safety than those of MTBE. TAEE could present an attractive alternative to MTBE if our findings regarding its efficacy and safety can be consistently reproduced in further studies.
Methods : The in vitro dissolubility was determined by measuring the dry weights of human gallstones after treating with each solvent. The in vivo dissolubility was determined by comparing the weights of solvent-treated gallstones and control (dimethyl sulfoxide)-treated gallstones, after the direct infusion of each solvent into the gallbladder of hamsters with gallstones.
Results : The in vitro results demonstrated a 24-h TAEE-dissolubility of 76.7%, 56.5 and 38.75% for cholesterol, mixed, and pigmented gallstones, respectively, which represented a 1.2-, 1.4-, and 1.3-fold increase in dissolubility compared to that of MTBE. In the in vivo experiment, the 24 h-dissolubility of TAEE was 71.7% and 63.0% for cholesterol and pigmented gallstones, respectively, which represented a 1.4- and 1.9-fold increase in dissolubility compared to that of MTBE. In addition, the cell viability assay and western blot analysis indicated that TAEE had a lower toxicity towards gallbladder epithelial cells than MTBE.
Conclusions : TAEE has higher gallstone dissolubility properties and safety than those of MTBE. TAEE could present an attractive alternative to MTBE if our findings regarding its efficacy and safety can be consistently reproduced in further studies.
SESSION
BP Poster Presentation 1
E-Session 7/27 ~ 7/29 ALL DAY